Membrane filter technique for the isolation of Yersinia enterocolitica
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چکیده
منابع مشابه
Membrane filter technique for the isolation of Yersinia enterocolitica.
A membrane filter procedure was developed for the isolation of Yersinia enterocolitica from aquatic environments. Primary differentiation was based on the fermentation of sorbitol, the absence of lysine decarboxylase and arginine decarboxylase-dihydrolase activities, and the production of urease. Sodium deoxycholate was incorporated as an inhibitor of background organisms. The presumptive ident...
متن کاملComparison of Enrichment Schemes for the Isolation of Yersinia Enterocolitica
Discussion: Considering the effect of sample weight on the detection of Salmonella bacteria (Davies et al., 2000), we cannot directly compare the three enrichment media used herein. However, we suggest the use of M1 whenever possible to obtain large amounts of fecal material (10g). Furthermore, the use of direct enrichment of swine feces in SC is discouraged. The coupling of RV to PCR was signi...
متن کاملIsolation of Yersinia enterocolitica from swine.
Data pertaining to 20 strains of Yensinia enterocolitica isolated from the cecal content of swine slaughtered at Toronto Abattoirs are presented. Fifteen of 20 cultures belonged to the two predominant human serotypes in Ontario (i.e., serotype O:3 and O:5,27.) Seven cultures of Y. enterocolitica O:3 belonged to the "Canadian" human phage type 9b. These findings are further proof that swine are ...
متن کاملDirect isolation of Yersinia enterocolitica and Yersinia pseudotuberculosis from meat.
Studies were done to determine the usefulness of dilute alkali (KOH) treatment of meat samples for direct isolation of Yersinia enterocolitica and Yersinia pseudotuberculosis, without enrichment. Virulent Y. enterocolitica and Y. pseudotuberculosis in pork contaminated with 10(2), 10(3), and 10(4) cells per g survived the direct KOH treatment and were never recovered by using KOH postenrichment...
متن کاملYersinia enterocolitica.
Characteristics of the disease Infectious dose is 10–100 organisms Incubation period is 1⁄2–4 (usually 1–3) days; up to a week for S. dysenteriae 1 Illness: diarrhea with fever and nausea; sometimes toxemia, vomiting, cramps, and tenesmus; dysentery—blood and mucus in stools; may cause hemolytic uremic syndrome; most severe in infants; mild and asymptomatic infections occur Duration: 4–7 days; ...
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ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 1982
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.43.4.829-834.1982